Extended Data Fig. 5: Effect of depletion of VPS35, LRRK2 and GSDME on lysosome size.
From: MAPL regulates gasdermin-mediated release of mtDNA from lysosomes to drive pyroptotic cell death
(a, b) Immunoblotting confirming knockdown of target genes in U2OS cells (a) VPS35, (b) GSDME and LRRK2 using siRNA. Non-targeting siRNA (siNT) was used as a control (c) MAPL overexpression causes a RING-dependent increase in lysosomal size rtTA, MAPL or ΔRING were expressed in U2OSOCT–GFP cells for 48 h before assessment of lysosomal size. N = 3 independent experiments.
