Extended Data Fig. 6: Overview of screening and identification of potential regulators of bivalent domains.

a. Schematic framework for the assessment and screening of factors regulating bivalent domains. See methods for details. b. Quantification of blastocyst formation rates following candidate factor screening. Data are presented as mean ± s.d. Source numerical data are available in source data. c. Number of differentially expressed genes in E5.0 EPI following knockout of candidate factors. d. Bubble plots showing the changes of individual gene sets analyzed by GSEA in E5.0 EPI following knockout of individual candidate factors. Color represents NES; bubble Size indicates adjusted P values determined by a one-sided permutation-based method with Benjamini-Hochberg correction. e. Heatmap showing the expression of pluripotency marker genes in E5.0 EPI from WT, Otx2^−/−, and Tfap2c^−/− embryos. f. Scatter plots showing H3K27me3 signals at the promoters of H3K27me3-only genes in E5.0 EPI from WT, Otx2^−/−, and Tfap2c^−/− embryos. The blue line represents fitted linear regression line. P-values were determined by two-sided Wilcoxon test without multiple testing correction. Source numerical data are available in source data.