Extended Data Fig. 7: Impaired resolution of TB-marked genes in ZBTB17-depleted EPIs during peri-implantation development.

a. PCA of transcriptomes comparing wild-type EPI samples across developmental stages (E3.5 to E5.5) and Zbtb17^−/− EPI at E4.5 and E5.0. b. Heatmaps (left) and box plots (right) of H3K27me3 enrichment at the promoters of TB-marked genes in E4.5 EPI from WT and Zbtb17^−/−embryos. Box plots show normalized H3K27me3 CUT&Tag signal intensity. P values were calculated using two-sided Wilcoxon rank-sum test. c. Heatmaps showing the expression of pluripotency-associated genes in EPI from WT and Zbtb17^−/−embryos at E4.5 and E5.0. d. IGV genome browser views showing H3K27me3 and H3K4me3 enrichment at pluripotency-associated genes in EPI from WT and Zbtb17^−/− embryos at E4.5 and E5.0. e. Heatmaps of H3K4me3 and H3K27me3 enrichment at the promoters of TB-marked genes in the EPI of WT and Zbtb17^−/−embryos across peri-implantation stages. f. Box plots quantifying H3K4me3 and H3K27me3 enrichment at the promoters of TB-marked genes in the EPI of WT and Zbtb17^−/− embryos. P values were calculated using two-sided Wilcoxon rank-sum test. g. PCA of H3K27me3 profiles comparing WT EPI samples across developmental stages (E3.5 to E5.5) and Zbtb17^−/− EPI at E5.0. h. Bubble plot showing statistically significant enrichment between up- and down-regulated genes in E5.0 EPI treated with dTAG-13 and bivalent or pluripotency-associated gene sets. Bubble color indicates P value determined by two-sided hypergeometric test without multiple testing correction; bubble size reflects the number of overlapping genes. Source numerical data are available in source data.