Extended Data Fig. 8: Genome-wide distribution of ZBTB17 and its interacting factors.

a. Heatmap showing expression of pluripotency genes in Zbtb17^dTAG/dTAG mESCs treated with DMSO, dTAG13, or dTAG13 + EED226 during transition from 2i/LIF to FA medium at 0, 12, 24, and 48 h. b. Pie charts showing (left) the proportion of genes activated after 48 h of pluripotency transition among 844 validated TB-marked genes (confirmed by ESC reChIP), and (right) the expression changes of these activated genes following ZBTB17 degradation. c. ZBTB17-binding motif identified in ESC ZBTB17 ChIP-seq peaks using HOMER. d. Genomic distribution preference of ZBTB17 ChIP-seq peaks. e. Box plot showing changes in EED levels at TB-marked and H3K27me3-only gene promoters after ZBTB17 degradation for 48 h, grouped by ZBTB17 promoter-binding intensity (1 = lowest, 5 = highest). P-values were calculated using one-sided Jonckheere–Terpstra test to assess the continuous trend of changes between groups. f. Box plot showing changes in H3K27me3 and H3K4me3 levels at H3K27me3-only gene promoters after 48 h of ZBTB17 degradation, and KDM6A/B enrichment at the same regions in ESCs, grouped by ZBTB17 promoter-binding intensity (1 = lowest, 5 = highest). P-values were calculated using one-sided Jonckheere–Terpstra test to assess the continuous trend of changes between groups. g. Comparison of KDM6A and KDM6B enrichment at ZBTB17-bound versus unbound promoters. All box plots represent the interquartile range (IQR), with the lower and upper bounds indicating the 25th and 75th percentiles, respectively. The median is shown by the line inside the box. Whiskers extend to the minimum and maximum values within 1.5 times the IQR. Source numerical data are available in source data.