Extended Data Fig. 10: Bivalency changes following ZBTB17 depletion during the pluripotency transition of human ESCs.

a. Morphology of ZBTB17 knockout and control hEPSCs and primed hESCs. Experiments were repeated three times using independent biological samples. Scale bar, 20 μm. b. Bar plot showing Gene Ontology enrichment analysis of differentially expressed genes between ZBTB17 knockout and control primed hESCs. P-values were determined by two-sided hypergeometric test without multiple testing correction. c. Box plots showing gene expression levels across primed-specific, hTB-, and hCB-marked gene sets in ZBTB17 knockout and control primed hESCs. P-values were calculated using two-sided Wilcoxon test. d. Heatmap showing the expression of human primed-state marker genes in ZBTB17 knockout and control primed hESCs. e. Boxplots comparing changes in gene expression, H3K27me3, and H3K4me3 levels among overactivated, unchanged, and silenced hTB-marked genes upon ZBTB17 knockout. f. IGV browser views showing changes in H3K27me3 and H3K4me3 at silenced hTB-marked genes in ZBTB17 depleted primed hESCs. g. GO of repressed hTB genes following ZBTB17 depletion involved in differentiation and gastrulation. P-values were determined by two-sided hypergeometric test without multiple testing correction. All box plots represent the interquartile range (IQR), with the lower and upper bounds indicating the 25th and 75th percentiles, respectively. The median is shown by the line inside the box. Whiskers extend to the minimum and maximum values within 1.5 times the IQR. Source numerical data are available in source data.