Fig. 2: Totipotent-like cell-derived aggregates resemble mouse early embryos from the 4-cell to 64-cell embryo stages. | Nature Cell Biology

Fig. 2: Totipotent-like cell-derived aggregates resemble mouse early embryos from the 4-cell to 64-cell embryo stages.

From: A continuous totipotent-like cell-based embryo model recapitulates mouse embryogenesis from zygotic genome activation to gastrulation

Fig. 2

a,b, Immunofluorescent analysis showing the expression of NR5A2 and TFAP2C in cell aggregates at S2 (a) and E2.5 embryos (b). n = 6 S2 cell aggregates from 2 experiments, n = 3 E2.5 embryos from 2 experiments. Scale bars, 20 μm. c, Feature plots showing the expression of genes regulated by Nr5a2 and Tfap2c in subpopulation 2 from S1 and cell aggregates at S2. Genes regulated by Nr5a2 and Tfap2c were identified using published bulk transcriptomic datasets (GSE229740 and GSE216256). d, Dot plots showing the average levels and proportion of cells expressing stage-specific marker genes in cell aggregates at S2. Developmental stage-specific marker genes for zygotes, 2-cell, 4-cell, 8-cell and 16-cell embryos were identified using published single-cell transcriptomic datasets (GSE136714). Expressions of these marker genes in zygotes, 2-cell, 4-cell, 8-cell and 16-cell embryos are shown as the control. e, UMAP analysis of transcriptome of totipotent-like cell-derived cell aggregates at S2. These were integrated with published single-cell transcriptomic datasets from early mouse embryo stages from zygotes to 16-cell embryos (GSE136714). f, Hierarchical clustering of cell aggregates at S2 and early mouse embryo stages from zygotes to 16-cell embryos (GSE136714). g,h, Immunofluorescent analysis showing the expression of OCT4, CDX2 and SOX17 in cell aggregates at S3 (g) and E3.5 embryos (h). n = 6 S3 cell aggregates from 2 experiments, n = 3 E3.5 embryos from 2 experiments. Scale bars, 20 μm. i, Dot plots showing the average levels and proportion of cells expressing stage-specific marker genes in cell aggregates at S3. Developmental stage-specific marker genes for zygotes, 2-cell, 4-cell, 8-cell, 16-cell, 32-cell and 64-cell embryos were identified using published single-cell transcriptomic datasets (GSE136714 and GSE84892). Expressions of these marker genes in embryos from zygotes to 64-cell embryos are shown as the control. j, UMAP analysis of transcriptome of totipotent-like cell-derived cell aggregates at S3. These samples were integrated with published single-cell transcriptomic datasets of early mouse embryo stages from zygotes to 64-cell embryos (GSE136714 and GSE84892). k, Hierarchical clustering of cell aggregates at S3 and early mouse embryo stages from zygotes to 64-cell embryos (GSE136714 and GSE84892).

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