Extended Data Fig. 8: PMLΔRBC promote polyG inclusion clearance in vivo and in vitro.
From: PML targets and resolves structured protein inclusions to mitigate neurodegeneration
a, Immunoprecipitation of HA-tagged PMLΔRBC from HEK293T cells co-expressing polyG–eGFP. b, Coomassie staining of purified WT and KR full-mutant PMLΔRBC-MBP-His proteins. c, d, Co-incubation with PMLΔRBC-MBP-His markedly decreased the formation of polyG aggregates (c) and reduced insoluble polyG (d) (polyG60×-eGFP, 10 μM; PMLΔRBC-MBP-His, 10 μM; 37 °C, 12 h). e, The SRS2 domain alone failed to remove polyG inclusions. f, g, Lysine-to-arginine (KR) mutations of PMLΔRBC suppressed polyG inclusion removal in a mutation number-dependent manner.
