Extended Data Fig. 10: mPMLΔRBC dissolves cytoplasmic TDP-43 and polyQ aggregates.
From: PML targets and resolves structured protein inclusions to mitigate neurodegeneration
a, Representative fluorescence images (left) and quantification (right) of TDP-43-CTF-mRuby3-NES aggregates in HEK293T cells expressing HA or mPMLΔRBC-HA. Data are presented as mean ± s.d. n = 6 fields of view per group collected across two independent experiments. Statistical significance was assessed using a two-tailed unpaired Student’s t-test. b, Coomassie staining of purified SUMO-mEm-TDP-43-His protein. c, Coomassie staining of purified mPMLΔRBC-MBP-His protein. d, Schematic of the sedimentation analysis of matured SUMO-mEm-TDP-43 fibres (20 μM, 37 °C, 24 h) treated with mPMLΔRBC-MBP-His (10 μM, 37 °C) for the indicated durations. e, mPMLΔRBC-MBP-His progressively dissolved pre-formed SUMO-mEm-TDP-43 fibres shown in d. f, Immunoblot analysis of Flag–TDP-43-CTF–eGFP–NES in fractions from HEK293T cells co-expressing mPMLΔRBC-HA with or without TAK-981 treatment (SUMOylation inhibitor, 500 μM, 6 h). g, Representative immunofluorescence images of htt72Q–KR-His and mPMLΔRBC in HEK293T cells. Line profiles show fluorescence intensities of each protein along dashed arrows.
