Extended Data Fig. 2: LCs are interdigitated with tMacs in adult testes.
From: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis
a, Distance between LCs and tMacs, Stromal cells, Myoid cells, and Sertoli cells in Fig. 1i (n = 50 LCs from 3 mice). b, Illustration of tissue clearing and imaging of testes from Cyp17a1Cre; R26tdTomato; Cx3cr1GFP mice. c, Bright field images of the whole testis before and after clearing from Cyp17a1Cre; R26tdTomato; Cx3cr1GFP mice. Scale bars, 2.5 mm. d, 3D reconstructed light-sheet images of the cleared whole testis, showing the distribution of LCs (tdTomato+) and tMacs (GFP+). Scale bars, 1 mm (left), 100 μm (middle), 60 μm (right). e, Representative confocal images and 3D reconstructions of the cross section of the testis from Cyp17a1Cre; R26tdTomato; Cx3cr1GFP mice (n = 3 mice). The testes sections were counterstained with DAPI. Scale bars, 500 μm (left), 50 μm (middle), 10 μm (right). f, Flow cytometry analysis showing the percentage of tdTomato+ particles within CD45+CD11b+F4/80+ tMacs in saline-treated (Con) or hCG-treated mice (hCG); WT were used as a negative control (n = 5 mice per group). Data are represented by mean ± SEM (a). Significances were determined using Kruskal–Wallis test (a). Schematic in b created in BioRender. Xia, K. (2026) https://biorender.com/wurmnz4. Source numerical data are provided.
