Extended Data Fig. 3: Isolation and characterization of LC-EVs.
From: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis
a, Experimental design for purifying LC-EVs by serial centrifugation and FACS. b, The gating strategy for sorting LC-EVs by FACS in the 3,000g pellets from Cyp17a1Cre; R26tdTomato mice. c, Representative images of pellets from the 50, 300, 650, and 3,000g fractions, respectively (n = 3 biological replicates). Scale bars, 50 μm (50g, 300g, and 650g), 2 μm (3,000g). d, Experimental strategy to label LCs membranes by intratesticular injection of AAV-DIO-Lck–EGFP into the testes of Cyp17a1Cre; R26tdTomato mice. e, Representative confocal images of the testes from AAV-DIO-Lck–EGFP-injected Cyp17a1Cre; R26tdTomato mice. The testes sections were counterstained with DAPI (n = 5 mice). Scale bar, 50 μm. f, Percentage of LCs infected with AAV-DIO-Lck–EGFP (n = 5 mice). Data are represented as mean ± SEM (f). Schematics in a,d created in BioRender. Xia, K. (2026) https://biorender.com/xlb1vjy. Source numerical data are provided.
