Extended Data Fig. 8: Isolation and characterization of tMac-EVs.
From: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis
a, Experimental design for enriching and purifying tMac-EVs by serial centrifugation and FACS. b, The gating strategy for sorting tMac-EVs by FACS in the 3,000g pellets from Cx3cr1GFP mice. c, Representative images of pellets from the 50g, 300g, 650g, and 3,000g fractions, respectively. Scale bars, 50 μm (50g, 300g, and 650g) and 5 μm (3,000g) (n = 3 biological replicates). d, Experimental strategy to label tMacs with membrane-targeted GFP in Cx3cr1CreER; mTmG mice. e, Representative confocal image and 3D reconstruction of tMac-EVs (with mitochondria) within LCs. The testes sections from TAM-induced Cx3cr1CreER; mTmG mice were immunostained with TOMM20 and LCs marker (3β-HSD) (n = 3 mice). Scale bars, 5 μm. Inset shows boxed regions that are magnified. Scale bar, 1 μm. Schematics in a,d created in BioRender. Xia, K. (2026) https://biorender.com/2813hkn.
