Extended Data Fig. 9: Strategies for labelling mitochondria of LCs, sorting tMacs and MHCIIhi tMac-EVs.
From: An extracellular vesicle-mediated mitochondrial transfer network critical for testosterone synthesis
a, Experimental strategy to label LCs mitochondria by intratesticular injection of AAV-mito–mCherry into the testes of WT mice. b, Representative confocal images of the testes from AAV-mito–mCherry-injected WT mice (n = 4 mice). Scale bar, 30 μm. c, Percentage of LCs infected with AAV-mito–mCherry (n = 4 mice). d, The gating strategy for sorting CD206 hi tMacs and MHCIIhi tMacs from Cx3cr1CreER; R26mitoD2 mice. e, Experimental strategy to isolate MHCIIhi tMac-EVs in Cx3cr1GFP mice by FACS. f, Detection and quantification of the percentage of MHCIIhi tMac-EVs from Cx3cr1GFP mice by FACS (n = 5 biological replicates). g, Analysis of mitochondria signal in purified MHCIIhi tMac-EVs by FACS (n = 5 biological replicates). h, Testosterone levels in LCs culture medium after 6 h of treatment (n = 5 biological replicates). i, ATP levels of LCs from the 3 groups (n = 5 biological replicates). Data are represented as mean ± SEM (c,f–i). Significances were determined using one-way ANOVA (h,i). Schematics in a,e created in BioRender. Xia, K. (2026) https://biorender.com/22gt4hs. Source numerical data are provided.
