Extended Data Fig. 3: Preparation of antibody–DNA conjugates.
From: Selection of DNA-encoded chemical libraries against endogenous membrane proteins on live cells
a) The conjugation reaction scheme. b) Native PAGE analysis of the reaction. Lane 1, an anti-EGFR antibody standard; lane 2: the reaction mixture. Marker is based on unmodified antibody. c) The bands a–c in b) were purified and analysed with native PAGE (polyacrylamide gel electrophoresis). d) The unlabelled antibody and purified bands a–c were characterized with ESI-MS; the results confirmed that conjugate b was the mono-DNA–antibody conjugate.
