Extended Data Fig. 2: Additional data on toehold displacement of BP after the labelling of FR on HeLa cells.

a) Structures of BP-2 and CP-2 and the labelling scheme. Fluorescent imaging and flow cytometry were used to monitor a series of control experiments. b) Toehold displacement with a mismatched DP after labelling. Unlike the complementary DP shown in Fig. 3, the mismatched DP did not reduce cell fluorescence. c)-e) After toehold displacement with a complementary DP to remove the original BP, a series of control experiments were performed. c): with no FA on BP-3; d): with a mismatched BP-3; e): with free FA competitor (50-fold). Experimental conditions were the same as in Fig. 2 of the main text. f) The same labelling and toehold displacement experiments were performed with the CBS/CA-12 system. Flow cytometry histograms after labelling and before/after DP displacement are shown. Strong fluorescence reduction was observed.