Extended Data Fig. 6: Pharmacological targeting of iron-regulated processes.
From: CD44 regulates epigenetic plasticity by mediating iron endocytosis
a, Western blots of H3K9me2 in cells co-treated with OSM and DFO or TGF-β and DFO as indicated. n = 1 for primary cells. b, Western blots of proteins whose genes are regulated by H3K9me2 in cells co-treated with OSM and DFO or TGF-β and DFO as indicated. n = 1 for primary cells. c, Molecular structure of clickable deferasirox (cDFX) (left), fluorescence microscopy of labelled cDFX and the mitochondrial component Cyt c (right). Scale bar, 10 μm. d, αKG quantification assay of MDA-MB-468 cells co-treated with EGF and deferasirox (DFX). n = 3 technical replicates. e, Western blots of CD44 and H3K9me2 in MDA-MB-468 cells co-treated with EGF and DFX. Data representative of n = 3 biologically independent experiments throughout the figure unless stated otherwise. Bars and error bars, mean values ± s.d.
