Extended Data Fig. 2: CD44-mediated iron uptake is Hyal-dependent in distinct cell lines and primary cells.
From: CD44 regulates epigenetic plasticity by mediating iron endocytosis
a, Flow cytometry of RhoNox-M fluorescence in HMLER CD44 and TFRC ko clones and a MCF7 CD44 ko clone treated with Hyal. b, Flow cytometry of RhoNox-M fluorescence in primary cancer cells and primary T cells treated with Hyal. n = 1. c, Flow cytometry of RhoNox-M fluorescence in MDA-MB-468 cells treated with Hyal of varying molecular mass. d, and e, Flow cytometry of RhoNox-M in MDA-MB-468, primary lung CTC, HMLER CD44high and MCF7 cells treated with Hyal, hyaluronidase or blocking antibodies. n = 1. HMM Hyal (0.6-1 MDa) was used in a, b, d. Data representative of n = 3 biologically independent experiments for a and c.
