Fig. 2: Ribozyme ligation activity is enhanced in coacervates containing charge-interspaced peptides as compared to homopeptides. | Nature Chemistry

Fig. 2: Ribozyme ligation activity is enhanced in coacervates containing charge-interspaced peptides as compared to homopeptides.

From: Charge-density reduction promotes ribozyme activity in RNA–peptide coacervates via RNA fluidization and magnesium partitioning

Fig. 2

a, 12.5% polyacrylamide urea denaturing gels show that ligation activity is maintained up to a ratio of 30 a.a./nt for (RGG)4. Product formation is rapidly lost for R9, K9 and (RGG)5 coacervates. Ctrl, control. The red box indicates residual enzymatic activity after the onset of coacervation. b–e, Relative amount of ligated substrate by gel analysis (red curve) and enzyme fraction (blue curve) in the coacervate phase against amino acid-to-nucleotide ratio for R9 (b), K9 (c), (RGG)5 (d) and (RGG)4 (e). Enzyme concentration in the coacervate phase was fitted to a Hill equation. Data are shown as mean ± 68% confidence interval; n = 3. Open blue circles indicate individual experiments. Product formation was fitted to a Hill equation. Data are shown as mean ± 68% confidence interval; n = 3. Open red circles indicate individual experiments.

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