Extended Data Fig. 1: Arginine homopeptides and heteropeptides show greater stability under peptide excess.

a-c, Sample turbidity at 500 nm of (a) Rn, (b) Kn and (c) (RGG)n peptides against amino acid-to-nucleotide ratio. Samples contained 5 µM E and 6 µM S2. At least 3 arginines, 6 lysines and 4 RGG repeats are necessary to achieve phase separation under standard reaction conditions. Lysine peptides different from K9 led to dissolution of the phase under peptide excess, most strikingly in the case of poly-K. Rn and (RGG)n peptides formed stable phases. Data are shown as the mean ± 68% CI, open circles indicate individual experiments.