Fig. 2: Exquisite high stability for megadalton particles in Orbitrap-based native MS.
a, Native MS spectrum constructed from binned detected centroids for the IgG1-RGY oligomers (that is IgG, (IgG)2, (IgG)3, (IgG)4, (IgG)6) and the nanocontainer AaLS-neg, both recorded at similar settings. Ion signals of different particles are colour-coded as annotated. Dots indicate charge states selected for survivability analysis. b, Comparing ion intensity between the first and second half of the transient is used as a proxy for ion survivability. This process is illustrated with ions that decay during different stages of the second half of the transient, resulting in varying ratios for the detected ion signals (black and green bars). c, Comparison of ion survival ratio for different charge states of different IgG oligomers and AaLS-neg using a colour code as in a. d, Favourable scaling of normalized travelled distance, centre-of-mass (COM) and CCS with increasing mass results in sharply reduced energy transfer per surface area, responsible for the plateau of stability reached for megadalton particles. Values on the vertical axis are shown relative to those for a hypothetical particle with a mass of 100 kDa.
