Extended Data Fig. 3: Evolution of FAD redox states in MmCPDII. | Nature Chemistry

Extended Data Fig. 3: Evolution of FAD redox states in MmCPDII.

From: Serial crystallography captures dynamic control of sequential electron and proton transfer events in a flavoenzyme

Extended Data Fig. 3

(a) In crystallo spectra of the oxidized (black line), neutral semiquinone (grey line), and fully reduced (dotted black line) states adopted by MmCPDII crystals. The occupancies of 100% for the oxidized and reduced states, as well as 65% for the neutral semiquinone state, were estimated by use of equations 1-3. (b) Projected occupancy of the FAD•- species over time. The projection was calculated by integrating a differential equation system resulting from previously published time constants and a quantum yield of 48%10. Numerical integration was performed using the Tinkercell software68. Integration was performed from 100 ps to 5 ms.

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