Extended Data Fig. 1: O-GlcNAcylation of SynGAP.
From: O-GlcNAcylation modulates liquid–liquid phase separation of SynGAP/PSD-95
a,b, Bar graph showing relative O-GlcNAcylation levels in Fig. 1c (a) and Fig. 1d (b). In a and b, the relative O-GlcNAcylation levels are normalized to that of GFP-SynGAP-α1 with no OGT overexpression and that of GFP-SynGAP CC-PBM with no OGT overexpression, respectively. Error bars represent mean ± SD. Results are from three independent experiments. Unpaired two-tailed Student’s t-test with 95% CI was used to determine difference, p < 0.05 is considered significant. c,d, ETD-MS/MS spectra of two O-GlcNAcylated peptides of recombinant GFP-SynGAP CC-PBM purified from HEK293T co-expressing OGT. The c2 ion unambiguously confirms O-GlcNAcylation at S1159 (c). The c12 and c14 ions unambiguously confirms O-GlcNAcylation at T1306 (d). The matched fragment ions are marked.
