Extended Data Fig. 2: SDS PAGE analysis of recombinant SynGAP CC-PBM, EPL-synthesized SynGAP CC-PBM, SynGAP-S1159OG, and SynGAP-T1306OG.
From: O-GlcNAcylation modulates liquid–liquid phase separation of SynGAP/PSD-95
SynGAP-T1306OG exhibited a slightly higher molecular weight than SynGAP-S1159OG, which can be attributed to the four residual amino acids at the N-terminus of SynGAP-T1306OG. The recombinant and EPL-synthesized SynGAP CC-PBM shown here contain the four amino acids. Of note, the four residual amino acids do not affect the LLPS behaviour of SynGAP CC-PBM/PSD-95 PSG (data not shown). Representative results are shown from three independent experiments.
