Extended Data Fig. 3: Receptor-binding studies of SST incubated with Aβ40. | Nature Chemistry

Extended Data Fig. 3: Receptor-binding studies of SST incubated with Aβ40.

From: Conformational and functional changes of the native neuropeptide somatostatin occur in the presence of copper and amyloid-β

Extended Data Fig. 3

a, Change in the fluorescence of the GRABSST sensor upon addition of SST treated with Aβ40 in the absence and presence of metal ions. Data are presented as mean ± s.e.m. (standard error of the mean); n = 19–21 per group. b, Fluorescence measurements of the GRABSST sensor after administration of metal-added and metal-free Aβ40 without SST. Data are presented as mean ± s.e.m. (standard error of the mean); n = 10 per group. Conditions: [SST] = 0.5 μM; [MCl2] = 0.5 μM; [Aβ40] = 0.5 μM; 20 mM HEPES, pH 7.4, 150 mM NaCl; 24 h incubation; constant agitation. The vertical lines indicate time points when the ΔF/F0 values were quantitatively analyzed and compared between groups.

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