Extended Data Fig. 8: Numerical simulation of the time-dependent assembly of a 3C system.
From: Functional advantages of building nanosystems using multiple molecular components
Using the rate constants measured from Fig. S13 and Fig. S14, we can predict the kinetic traces of assembly of the dimer (plain line) and the trimer (dashed line) using numerical simulation. This enables us to test different concentration conditions before experimentation. (a) Changing [A] only affects dimer assembly while (b) changing [B] and [C] affects the formation of the trimer. This illustrates that the limiting step of trimer assembly, in these conditions, is the formation of the productive dimer BC. These results agree with our experimental data (Fig. 4b in main text and Fig. S16 and Fig. S17). Numerical simulations are performed using MATLAB®.
