Extended Data Fig. 2: Thermal denaturation analyses of the 1C, 2C and 3C systems.

(a) Thermal denaturation profiles of the 1-component (black), 2-component (blue) and 3-component systems (green). (b) Left: Van’t Hoff analysis of the thermal denaturation curves enables the extraction of the thermodynamic parameters ΔH°_Ass and ΔS°_Ass (see panels c) and d), respectively). Right: extrapolation of the ΔG°_Ass at 23˚C (right side) are in good agreement with the values obtained using urea denaturation. (c, d) As observed for the m-values and ΔG°_Ass (see Fig. S3), ΔH°_Ass and ΔS°_Ass are also linearly dependant on the number of base pairs broken/formed in the assembly/disassembly transition. ΔH°_Ass = −60 ± 1 kcal·mol⁻¹, −85.8 ± 0.8 kcal·mol⁻¹, and −130 ± 7 kcal·mol⁻¹, for the 1C, 2C, and 3C system, respectively. ΔS°_Ass = −179 ± 4 cal·mol⁻¹·K⁻¹, −241 ± 3 cal·mol⁻¹·K⁻¹, and −355 ± 22 cal·mol⁻¹·K⁻¹ for the 1C, 2C, and 3C system, respectively. All experiments are performed in PBS buffer (10 mM NaH₂PO₄, 40 mM NaCl, pH = 7.00). Data and errors are presented as the values obtained from the Van’t Hoff linear regression (n = 1).

Source data