Extended Data Fig. 1: Photoactivation of split UnaG with spatiotemporal precision in living cells through intracellular LASL.
From: Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation
a-c, Mask-based photolithography spatiotemporally directs UnaG reassembly within HEK-293T cell culture. a, Fluorescent images of culture dish with inlays of exposure boundary magnified. b, Individual cell UnaG/mCh signal quantified radially outwards from the photomask’s center, normalized to the average UnaG/mCh ratio in unexposed cells. Dashed line indicates exposure edge. c, Violin scatter plots of normalized UnaG/mCh ratios in light-(un)exposed regions. Light treatments, λ = 365 nm, 20 mW cm−2, 20 min. Asterisks denote conditions with statistically significant differences in signal (p < 0.0001, two-tailed unpaired t-tests). Similar results were independently achieved in 3 experimental replicates. Scale bars, 1 mm (a).
