Fig. 4: Processing of 3a and 3b by CgnD and CgnL.
From: Unusual peptide-binding proteins guide pyrroloindoline alkaloid formation in crocagin biosynthesis
a, In vitro biosynthesis of the crocagin A core scaffold, 4a, using CgnD. m/z represents mass divided by charge number and the horizontal axis in a mass spectrum is expressed in units of m/z. b, Surface representation of the CgnD dimer. Active sites are highlighted by dashed boxes. c, N-methylation of 4a by CgnL to yield 5a. d, Cartoon representation of the CgnL structure. S-adenosylhomocysteine is shown as black sticks. Details of a and c can be found in Supplementary Figs. 18 and 21. Representative experiments were repeated independently at least three times with similar results.
