Extended Data Fig. 1: LLPS regime of α-Syn inside microemulsion droplets.
From: Mass photometric detection and quantification of nanoscale α-synuclein phase separation
a. (Left) Schematic of a 3-component microemulsion droplet maker (materials and methods). The inlets for protein, PEG and FC-40 + surfactant are marked in blue. The outlet for microemulsion droplet collection is marked in grey. The arrows indicate direction of flow. (Right) A representative bright-field (BF) microscopy image of the region where the individual components mix is shown. b. Representative fluorescence microscopy images of the spontaneous LLPS regime of α-Syn inside the microemulsion droplets. The blue dashed circles mark the boundary of the microemulsion droplets. The white triangular pointer and the enlarged inset (right) indicate spherical phase-separated α-Syn assemblies inside the microemulsion droplets. The experiment is repeated n = 2 independent times with similar observations. c. Comparison of LLPS regimes of α-Syn using the sealed well-plate method (left) and inside microemulsion droplets (right). The dashed box (left) represents the conditions which are replicated inside microemulsion droplets. The blue circles indicate LLPS and the red circles indicate no LLPS/soluble. PEG: PEG-8000 unless mentioned otherwise.
