Extended Data Fig. 1: Reaction conditions of RNA cloaking with acylimidazole reagents.
A heatmap shows reaction conditions for each acylimidazole reagent (1-7) required to introduce the desired level of RNA cloaking using a model 18nt tRF-3005 RNA. The X-axis indicates the number of 2′-acyl adducts per RNA molecule identified by MALDI-TOF. The color intensity of each cell represents the mass intensity of the acyl adduct peak (numbers at bottom show numbers of acyl adducts for the model RNA strand). See Supplementary Table 1 for quantification.
