Extended Data Fig. 4: qPCR independently validates the transcriptome interactions of Levofloxacin (Lev) at 15 transcriptome binding sites in HEK293 cells.
From: Pervasive transcriptome interactions of protein-targeted drugs
Workflow showing the strategies of validating competable 2´-OH acylation sites with qPCR (Top panel). qPCR validated and quantified the relative level of 2´-OH acylation at the drug-binding loci. Data represent mean ± s.e.m., n = 3 biologically independent experiments. Statistical significance was calculated with two-tailed unpaired Student’s t-tests: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. P values are 0.0227 (FAM71F2), 0.0007 (EEF2), <0.0001 (RPL5), 0.0346 (RNU1-88P), 0.0015 (H3F3B), 0.0023 (ARPC3), 0.0009 (YBX1), 0.0019 (SIAE), 0.0478 (RAN), 0.0006 (NME1), <0.0001 (ILF2), 0.0055 (XR_934306.3), 0.0051 (SIK3), 0.0140 (FTL), and 0.0103 (SNORD110).
