Extended Data Fig. 3: Chiral specificity of trypsin digestion of ribosomal protein L25 and Dpo4. | Nature Chemistry

Extended Data Fig. 3: Chiral specificity of trypsin digestion of ribosomal protein L25 and Dpo4.

From: Mirror-image trypsin digestion and sequencing of D-proteins

Extended Data Fig. 3

ac, Analytical RP–HPLC chromatograms of the synthetic d-L25 before (a) and after digestion by the recombinant l- (b) and synthetic l-trypsin (c). d,e, Analytical RP–HPLC chromatograms of the synthetic l-L25 before (d) and after digestion by the synthetic d-trypsin (e). fh, Analytical RP–HPLC chromatograms of the synthetic d-Dpo4-5m before (f) and after digestion by the recombinant l- (g) and synthetic l-trypsin (h). i,j, Analytical RP–HPLC chromatograms of the recombinant l-Dpo4-5m before (i) and after digestion by the synthetic d-trypsin (j). The experiments were performed twice with similar results.

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