Extended Data Fig. 4: Cleavage site specificity of trypsin digestion of ribosomal protein L25 and Dpo4.
From: Mirror-image trypsin digestion and sequencing of D-proteins

a, Observed cleavage frequency at the P1 site of l-L25 digested by the recombinant l- and synthetic l-trypsin, and of d-L25 by the synthetic d-trypsin. b, Observed cleavage frequency at the P1 site of l-Dpo4-5m digested by the recombinant l- and synthetic l-trypsin, and of d-Dpo4-5m by the synthetic d-trypsin. c, Observed cleavage frequency of l-L25 digested by the recombinant l- and synthetic l-trypsin, and of d-L25 by the synthetic d-trypsin, at trypsin cleavage sites with lysine at the P1 site and with or without proline at the P1′ site, displayed on a log scale. d, Observed cleavage frequency of l-Dpo4-5m digested by the recombinant l- and synthetic l-trypsin, and of d-Dpo4-5m by the synthetic d-trypsin, at trypsin cleavage sites with lysine at the P1 site and with or without proline at the P1′ site, displayed on a log scale. The experiments were performed twice with similar results.