Extended Data Fig. 4: Testing droplet-like behaviors of ASNS condensates and LTA4H condensates.
From: High-throughput and proteome-wide discovery of endogenous biomolecular condensates
a, Time-dependent images showed the liquid-like fusion and splitting behaviors of the ASNS-GFP condensates. b, Fluorescent image of ASNS-GFP condensates. The dash line circle indicated the area of nucleus. c, The percentage of ASNS-GFP in the condensed form as compared to the total cellular protein. n = 7 independent experiments, OE represents overexpression. d-e, Quantification (d) and sequential images (e) of FRAP assays on ASNS-GFP condensates showed the recovery of ASNS-GFP after photo-bleaching. n = 6 independent experiments. f, Time-dependent images showed the liquid-like fusion and splitting behaviors of the LTA4H-GFP condensates. g, Fluorescent image of LTA4H-GFP condensates. The dash line circle indicated the area of nucleus. h, The percentage of LTA4H-GFP in the condensed form as compared to the total cellular protein. n = 7 independent experiments, OE represents overexpression. i-j, Quantification (i) and sequential images (j) of FRAP assays on LTA4H-GFP condensates showed the recovery of LTA4H-GFP after photo-bleaching. n = 6 independent experiments. k, Numbers of detected proteins of 8 types of known membraneless condensates in the detected condensate proteins upon the short-term treatment of TGF-β. In c, d, h, i, data are means ± SD and were analysed by two-sided unpaired student’s t test.
