Extended Data Fig. 1: In situ imaging confirmed that the isolated condensates remained undissociated upon the treatment of the RIPA or NP-40 lysis buffer.
From: High-throughput and proteome-wide discovery of endogenous biomolecular condensates
a, Cells were transfected YBX1-EGFP to form YBX1-EGFP condensates in microfluidic channel. The RIPA buffer or NP-40 buffer was introduced into the cells by microchannels. b, YBX1-EGFP condensates remained for at least 40 mins with the treatment of NP-40 buffer. However, NP-40 was observed to insufficiently dissolve cell membrane and be unable to disrupt the nuclear membrane. c, YBX1-EGFP condensates remained for at least 40 mins with the treatment of RIPA buffer, while cellular lipid membrane and nuclear structure were sufficiently dissolved by RIPA buffer. Representative results from three independent experiments.
