Extended Data Fig. 3: Testing droplet-like behaviors of MRPL23 condensates and TOE1 condensates.
From: High-throughput and proteome-wide discovery of endogenous biomolecular condensates
a, Time-dependent images showed the liquid-like fusion and splitting behaviors of the MRPL23-GFP condensates. b, Fluorescent image of MRPL23-GFP condensates. The dash line circle indicated the area of nucleus. c, The percentage of MRPL23-GFP in the condensed form as compared to the total cellular protein. n = 7 independent experiments. d-e, Quantification (d) and sequential images (e) of FRAP assays on MRPL23-GFP condensates showed the recovery of MRPL23-GFP after photo-bleaching. n = 6 independent experiments. f, Time-dependent images showed the liquid-like fusion and splitting behaviors of the TOE1-GFP condensates. g, Fluorescent image of TOE1-GFP condensates. The dash line circle indicated the area of nucleus. h, The percentage of TOE1-GFP in the condensed form as compared to the total cellular protein. n = 10 independent experiments. i-j, Quantification (i) and sequential images (j) of FRAP assays on TOE1-GFP condensates showed the recovery of TOE1-GFP after photo-bleaching. n = 6 independent experiments. In c, d, h, i, data are means ± SD and were analysed by two-sided unpaired student’s t test.
