Extended Data Fig. 5: Material properties of A-IDP condensates.
From: Expanding the molecular language of protein liquid–liquid phase separation
(a) FRAP of WT and GRGNSPWS with RGG-GFP-RGG as a fluorescent tracer. Data are presented as mean values +/− SD, n = 4 (WT), n = 9 (GRGNSPWS) different condensates from one experiment. (b) Effect of total protein concentration on condensate viscosity, as measured by microrheology. Measurements were conducted for GRGNSPFS and GQGNSPYS, at two concentrations each. Data are presented as mean values +/− SD, n = 4 videos from one experiment. (c) MSDs measured in polycationic GRGNSPYS condensates using PEGylated and carboxylated beads. (d) Viscosity of GRGDSPYS (WT), GQGNSPYS, and GRGNSPYS determined by particle tracking microrheology of 0.5 μm PEGylated vs. carboxylated beads. Two factor with replication ANOVA confirmed difference in viscosities between 0.5 μm PEGylated and carboxylated beads is not statistically significant, with p-value of 0.753. Data are presented as mean values +/− SD, n = 4 videos from one experiment. (e) Viscosity of GRGDSPYS (WT), GQGNSPYS, and GRGNSPYS determined by particle tracking microrheology of 0.5 μm vs. 1 μm bead diameters. Two factor with replication ANOVA confirmed difference in viscosities between 0.5 μm and 1 μm beads is not statistically significant, with p-value of 0.268. Data are presented as mean values +/− SD, n = 4 videos from one experiment.
