Fig. 3: BCP. | Nature Chemistry

Fig. 3: BCP.

From: Discovery of isoquinoline sulfonamides as allosteric gyrase inhibitors with activity against fluoroquinolone-resistant bacteria

Fig. 3

a, Microscopy images of E. coli BW25113 from the BCP experiment. Conditions are, from top to bottom: DMSO (1.25%), AMP (12.5 µM), RIF (12.5 µM), TET (6.25 µM), CIP (0.0625 µM), LEI-801 (6.25 µM) and LEI-800 (6.25 µM). From left to right, the channels indicate DIC, DAPI, FM4-64 and DAPI/FM4-64 combined. The bacteria treated with LEI-801 displayed a phenotype that is consistent with the DMSO control, while incubation with LEI-800 led to a phenotype resembling the CIP and TET morphological profile. Scale bars, 10 μm. b, Individual graphs of six exemplary features extracted from BCP microscopy after image analysis. Each dot represents the measured value for an individual bacterium. c, PCA plot of all the morphological features extracted from three independent BCP experiments.

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