Extended Data Fig. 1: Summary of enzyme cascades involving SOI and the bacterial styrene-degradation pathway.

a, Isomerization of phenyl epoxides by membrane-bound SOI can be used to produce a variety of valuable compounds by involving other enzymes in cascade reactions. The high regio- and stereo-specificity of SOI is important for the synthesis of (S)- and (R)-configured alcohols, acids and amines. b, The styrene-degradation pathway comprises styrene monooxygenase (SMO), styrene oxide isomerase (SOI), and phenylacetaldehyde dehydrogenase (PAD). Styrene monooxygenases are two-component flavoproteins that catalyse the NADH and FAD-dependent enantioselective epoxidation of styrene to styrene oxide. Styrene oxide isomerase is a membrane-bound protein that catalyses the isomerization of styrene oxide to phenylacetaldehyde. Phenylacetaldehyde dehydrogenase catalyses the NAD⁺-dependent oxidation of phenylactealdehyde to phenylacetic acid. Phenylacetic acid catabolism leads to the production of succinyl-CoA and acetyl-CoA, which is then feed into the tricarboxylic acid (TCA) cycle.