Fig. 3: Characterization of fZ-motif structure by NMR.
From: A folding motif formed with an expanded genetic alphabet
a, 1H NMR spectra of ZZZ oligo at indicated pH in two indicated solvent systems. All samples contain 50 mM phosphate buffer. The asterisks represent DNA samples from which triethylamine was not fully removed by desalting. b, NOESY spectrum of ZZZ at pH = 8.5 in H2O/D2O (9:1). Box a (red) holds the cross-peak assigned to neighbouring [Z]N1H and [Z]NH2. Box b (blue) holds the cross-peak assigned to neighbouring [Z]N1H and [Z-]NH2. c, 1H NMR spectrum assignment of ZZZ at pH = 8.5 in D2O solution or in H2O/D2O (9:1), ZZZ at pH = 7 in D2O solution and free dZ over a range of pHs (6, 7, 8.5 and 9 in D2O). The primes indicate protons in sugar. Signal multiplicity is abbreviated as follows: s, singlet; d, doublet; t, triplet; m, multiplet; and br, broad.
