Extended Data Fig. 5: Analysis of the reactivity between H3K9NleS+me2 peptide (5) and peptide or proteins without binding pocket of H3K9me3. | Nature Chemistry

Extended Data Fig. 5: Analysis of the reactivity between H3K9NleS+me2 peptide (5) and peptide or proteins without binding pocket of H3K9me3.

From: Single-electron transfer between sulfonium and tryptophan enables site-selective photo crosslinking of methyllysine reader proteins

Extended Data Fig. 5

a, HPLC analysis of reaction mixture of H3K9NleS+me2 peptide (5) and a Tryptophan-containing short peptide (S3) under the standard crosslinking condition. Integration of the peptide (S3) peak did not change, and no crosslinked peptide product was observed. b-e, Mass spectrometry analysis of reaction mixture H3K9NleS+me2 peptide (5) and tryptophan-containing proteins under the standard crosslinking condition. Tryptophan residues are shown as stick in green.

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