Extended Data Fig. 10: Photo-induced crosslinking reaction between LgBiT and sulfonium-SmBiT.

a, Structural analysis of NanoBiT. All aromatic residues were highlighted in pink. It demonstrated that W11 is not in an aromatic cage. b, Interface between LgBiT and SmBiT indicates that W11 is on LgBiT surface rather than inside a pocket. c, Mass spectrometry analysis of LgBiT and sulfonium myc-SmBiT (S25) crosslinking.