Extended Data Fig. 3: Additional data of binding and crosslinking activity of H3K9NleS+me2 peptide to CBX1. | Nature Chemistry

Extended Data Fig. 3: Additional data of binding and crosslinking activity of H3K9NleS+me2 peptide to CBX1.

From: Single-electron transfer between sulfonium and tryptophan enables site-selective photo crosslinking of methyllysine reader proteins

Extended Data Fig. 3

a, Binding kinetics of interaction between CBX1 and H3K9NleS+me2 peptide (S16) by bio-layer interferometry, and steady-state graph is shown on the right. b, Mass spectrometry analysis of the crosslinking between CBX1 and H3K9NleS+me2 peptide (5) at time points. c,d, Mass spectrometry analysis of the crosslinking between CBX1 and peptide (5) by addition of TEMPO (7 mM) for 5 min or 30 min. e, Top-down mass spectrometry analysis of the methyl-CBX1 conjugate.

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