Extended Data Fig. 8: Polar interaction networks connecting static and switchable TMHs in the Hyd_high6 design and A2AR.

a, Cartoon representation of the Hyd_high6 receptor active state model where the adenosine ligand and side-chains in the vicinity of the designed mutations are highlighted in sticks. b,c, zoomed view on the designed region in Hyd_high6 (b) and A2AR (c). Polar contacts are represented with red dotted lines. Residues are numbered using the Ballesteros Weinstein notation. Y315 and E316 belong to the G-protein Gαs.