Fig. 4: Polar interaction networks connecting static and switchable TMHs in the Hyd_low1 design and A2AR.

a, Cartoon representation of the Hyd_low1 receptor active-state model, where the adenosine ligand and side chains in the vicinity of the designed mutations are highlighted as stick models. b,c, Enlarged views of the designed region in Hyd_low1 (b) and A2AR (c). Polar contacts are represented by red dotted lines. Residues are numbered using the Ballesteros–Weinstein notation.