Fig. 5: Restoration of interface rigidity.

a, Design of LR 3PS and comparison of local and global flexibility with the original short 3PS and long 3PS designs. b, Representative frames from oxDNA simulations of the 3PS, coloured by the average root mean square fluctuation (RMSF). c, Steady-state AFM of LR 3PS, showing a restored radial large network assembly. AFM z-colour bars are adjusted for optimal data presentation (the lowest intensity corresponds to background at 0 nm and the highest intensity corresponds to DNA at approximately 2 nm). d, ND of LR 3PS assemblies at decreasing concentrations ([x] = 3.8 nM); dashed lines represent references for short rigid (SR) and long flexible (LF) monomers. The box represents the IQR, with the lower and upper edges corresponding to the 25th and 75th percentiles. Whiskers extend to the smallest and largest data points within 1.5 times the IQR from the lower and upper quartiles, respectively. Outliers, defined as data points beyond this range, are shown as individual points. The AFM images of 1/2× and 1/3× are shown in Supplementary Fig. 28. Additional AFM images for all concentrations are shown in Supplementary Fig. 29. e, Polygon distributions for LR 3PS assemblies, using images from 12 regions across the mica surface, each with an area of 750 nm × 750 nm, for each condition tested. The error bars represent mean values ± s.d. f, An approximate phase diagram as a function of probability to be in the open state and the patch width for σ = 1 and δ = 0.038. Purple, short-like phase; orange, long-like phase; grey, gas phase. Each data point represents a set of conditions simulated.