Fig. 2: Engineering translation components for encoding mTX.
From: Efficient and selective energy transfer photoenzymes powered by visible light
a, An engineered variant of MjTyrRS (AcdRS2b) previously developed to encode the fluorescent amino acid Acd41 was re-engineered for mTX incorporation by introducing one mutation by directed evolution. b, Structural representation of MjTyrRS. Pink spheres highlight mutations previously introduced into MjTyrRS to afford AcdRS2b. The blue sphere indicates the L108W mutation introduced to improve mTX incorporation efficiency. Teal shading indicates tRNA binding poses and the red dotted outline indicates the amino acid binding pocket. c, Bar chart showing the relative fluorescence unit (RFU) values measured in a GFP 150 TAG expression assay with AcdRS2b and mTX-RS variants. Error bars represent the s.d. of independent triplicate measurements. d, Cultures expressing GFP 150 TAG in the presence and absence of mTX.
