Extended Data Fig. 3: Bioinformatic analysis of the putative HKASs. | Nature Chemistry

Extended Data Fig. 3: Bioinformatic analysis of the putative HKASs.

From: Structural insights into two thiamine diphosphate-dependent enzymes and their synthetic applications in carbon–carbon linkage reactions

Extended Data Fig. 3

a, Sequence alignment of ScyA and two candidate HKASs (CsmA and CsmB) from strain C. sp. PKU-MA01392. CsmA and CsmB share 26% and 24% sequence identities with ScyA, respectively. b, Phylogenetic analysis of CsmA, CsmB, and BbmA with known HKASs. CsmA, CsmB, and BbmA are shown in red, green, and blue, respectively. The coupling reaction types of these HKASs were labeled on the right of the enzyme names. MEGA 7.0 was used to construct the phylogenetic tree by maximum likelihood method. The PDB IDs or NCBI accession numbers of proteins are: 5FEM (ScAHAS), 6DEK(CaAHAS), 7EHE (ThAHAS), 6U9H (AtAHAS), 6LPI (EcAHAS), 1OZF (KpALS), 4RJJ (AlsS), WP_111325691.1 (ThZK0150), ALL53321.1 (NzsH), WP_012058936.1 (Cbei_2730), WP_012990202.1 (XclA), WP_063628719.1 (CybE) and WP_012408018.1 (ScyA) c, The encoding gene of CsmA and its neighboring genes. d, The encoding gene of CsmB and its neighboring genes that are responsible for branched-chain amino acid biosynthesis.

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