Extended Data Fig. 5: The conversion rates in type II coupling reactions catalyzed by CsmA, BbmA and the double mutants BbmAL112S/W288T.
(a)–(f), The total conversion rates of the two NaBH4 reduction products during the coupling reactions between IPA (S2) and HPPA (S3, a), PPA (S5, b), NOPA (S6, c), HMPPA (S7, d), OPBA (S8, e), or NPA (S9, f), respectively. g, The conversion rates in the homocoupling reactions of IPA (S2). (h)–(l), The total conversion rates of the two NaBH4-reduction products during the coupling reactions between HPPA (S3) and PPA (S5, h), NOPA (S6, i), HMPPA (S7, j), OPBA (S8, k), or NPA (S9, l), respectively. m, The conversion rates in the homocoupling reactions of HPPA (S3). All chromatograms are shown in the Supplementary Information (Supplementary Fig. 4). The conversion rates in percentile were determined from three original independent replicates of reactions (shown as dots) and the error bars were generated as mean values +/− SD (standard deviations) from the three independent replicates.
