Extended Data Fig. 7: Replication efficiency of template A upon iterative replication cycling. | Nature Chemistry

Extended Data Fig. 7: Replication efficiency of template A upon iterative replication cycling.

From: Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme

Extended Data Fig. 7

Left panels, Replication reactions were set up as in Fig. 2c with varying starting AD duplex concentrations and continued over 21 cycles (including 5 serial dilutions as shown in Fig. 2b). Right panels, Calculated strand amplification in these reactions. After x cycles, the concentration of primer reaching full-length (above that in the no template control) (= [≥FL(x)]) was measured by densitometry and normalized to the expected concentration of starting AD remaining after serial dilution: Amplification after x cycles = 1 + 2^((x-1)/4)*([ ≥ FL(x)] ÷ starting [AD]).

Source data

Back to article page