Fig. 4: Information propagation by sequence-specific catalytic dimerization.
a, The design of monomers to demonstrate accurate information propagation in catalytic dimerization. We consider three types of Mx, differentiated by their primary toehold, and three types of Ny, differentiated by their handholds. Fluorescent labelling, using poly(T) linkers of variable length, allows the identification of all MxNy complexes through gel electrophoresis. Templates Txy are intended to selectively template the formation of MxNy from a pool of all six monomer species. b, The fluorescent scan of gel electrophoresis demonstrating sequence-specific templating. Products control: the signal produced by each possible MxNy dimer produced by annealing 75 nM of each monomer. Templated reactions, the reaction mixture in which a low concentration of a single Txy (5 nM) is combined with 100 nM of each MxL monomer and 75 nM of each Ny monomer. The observed products and signal from unreacted monomers in each well after 40 h of reaction is consistent with the intended MxNy production. The false colours include: blue, Alexa 488; green, Alexa 546; red, Alexa 647; cyan, FRET 488/546; yellow, FRET 546/648; purple, FRET 488/648.
